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KMID : 0620920090410050341
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Experimental & Molecular Medicine
2009 Volume.41 No. 5 p.341 ~ p.348
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ICAM-1/LFA-1 interaction contributes to the induction of endothelial cell-cell separation: implication for enhanced leukocyte diapedesis
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Wee Hyun
Oh Hyun-Mee
Jo Jae-Hyeok
Jun Chang-Duk
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Abstract
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The basic route and mechanism for diapedesis has not yet to be fully defined. Here we present evidence that "cell-cell separation" between endothelial cells (ECs) may provide a route for leukocyte diapedesis. We unexpectedly found that extensive interaction between peripheral blood leukocytes and ECs that were activated by TNF-α induced the opening of EC contacts and, surprisingly, resulted in cell-cell separation. This event was specific to the intercellular adhesion molecules- 1 (ICAM-1)/leukocyte function- associated antigen- 1 interaction, as demonstrated by the following: (1) ICAM-1 expression correlated with increased EC contraction; and (2) the blocking of ICAM-1 selectively inhibited EC separation. Thus, we suggest that "cell-cell separation" could be a mechanism for diapedesis in situations that may require massive leukocyte infiltration.
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KEYWORD
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cell movement, endothelial cells, intercellular adhesion molecule-1, leukocytes, lymphocyte function-associated antigen-1
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